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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
17/12/2018 |
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Data da última atualização: |
17/12/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, J. S.; CHIARI, L.; RESENDE, R. M. S.; VILELA, M. de M.; SALGADO, L. R. |
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Afiliação: |
Juliana Santos Souza, Universidade Anhanguera - Uniderp/Campus Agrárias; LUCIMARA CHIARI, CNPGC; ROSANGELA MARIA SIMEAO, CNPGC; MARIANE DE MENDONCA VILELA, CNPGC; Leonardo Rippel Salgado, CNPGC. |
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Título: |
Development, Validation and Characterization of Genic Microsatellite Markers in Urochloa Species. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
American Journal of Plant Science, v. 18, n. 3, p. 314-319, July/Sept. 2018. |
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DOI: |
DOI: 10.4236/ajps.2018.92023 |
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Idioma: |
Inglês |
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Conteúdo: |
Urochloa (syn. Brachiaria ) cultivars represent 85% of tropical pastures occupying 114 million hectares of cultivated grasslands in Brazil. Despite the commercial importance of the Urochloa species, low molecular information is available and is far from saturating the genome. Investigating and obtaining more markers associated to characteristics of difficult and late expression can benefit in accelerating breeding programs of more important species. Aiming to increase the number of molecular markers, genic microsatellite markers were obtained from transcriptome of U. decumbens and analyzed for their cross-amplification to U. brizantha , U. humidicola and U. ruziziensis . Genic microsatellite markers were isolated from a transcriptome obtained of U. decumbens ?Basilisk? roots. Specific primers were designed for one hundred loci, and 32 were polymorphic presenting polymorphism informative content values ranging of 0.12 to 0.81 (mean 0.54). Amplified microsatellite regions yield an average of 4.44 alleles per locus (ranging of 1 to 13). Cross-amplification to U. brizantha , U. humidicola and U. ruziziensis were successfully performed, although the number of the loci transferred varied among them. Multiple orrespondence Analysis revealed three distinct groups separating accessions and species. Four genetic markers presented high potential to distinguish sexual and apomictic accessions of Urochloa and must be further investigated. The genic markers identified in this study are the first set of expressed sequence tagged molecular markers for Urochloa species. MenosUrochloa (syn. Brachiaria ) cultivars represent 85% of tropical pastures occupying 114 million hectares of cultivated grasslands in Brazil. Despite the commercial importance of the Urochloa species, low molecular information is available and is far from saturating the genome. Investigating and obtaining more markers associated to characteristics of difficult and late expression can benefit in accelerating breeding programs of more important species. Aiming to increase the number of molecular markers, genic microsatellite markers were obtained from transcriptome of U. decumbens and analyzed for their cross-amplification to U. brizantha , U. humidicola and U. ruziziensis . Genic microsatellite markers were isolated from a transcriptome obtained of U. decumbens ?Basilisk? roots. Specific primers were designed for one hundred loci, and 32 were polymorphic presenting polymorphism informative content values ranging of 0.12 to 0.81 (mean 0.54). Amplified microsatellite regions yield an average of 4.44 alleles per locus (ranging of 1 to 13). Cross-amplification to U. brizantha , U. humidicola and U. ruziziensis were successfully performed, although the number of the loci transferred varied among them. Multiple orrespondence Analysis revealed three distinct groups separating accessions and species. Four genetic markers presented high potential to distinguish sexual and apomictic accessions of Urochloa and must be further investigated. The genic markers identified in this study are th... Mostrar Tudo |
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Palavras-Chave: |
Signalgrass; Simple Sequence Repeat; Transferability. |
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Thesagro: |
Brachiaria. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/188572/1/8-Development-validation-and-characterization.pdf
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Marc: |
LEADER 02299naa a2200229 a 4500
001 2101685
005 2018-12-17
008 2018 bl uuuu u00u1 u #d
024 7 $aDOI: 10.4236/ajps.2018.92023$2DOI
100 1 $aSOUZA, J. S.
245 $aDevelopment, Validation and Characterization of Genic Microsatellite Markers in Urochloa Species.$h[electronic resource]
260 $c2018
520 $aUrochloa (syn. Brachiaria ) cultivars represent 85% of tropical pastures occupying 114 million hectares of cultivated grasslands in Brazil. Despite the commercial importance of the Urochloa species, low molecular information is available and is far from saturating the genome. Investigating and obtaining more markers associated to characteristics of difficult and late expression can benefit in accelerating breeding programs of more important species. Aiming to increase the number of molecular markers, genic microsatellite markers were obtained from transcriptome of U. decumbens and analyzed for their cross-amplification to U. brizantha , U. humidicola and U. ruziziensis . Genic microsatellite markers were isolated from a transcriptome obtained of U. decumbens ?Basilisk? roots. Specific primers were designed for one hundred loci, and 32 were polymorphic presenting polymorphism informative content values ranging of 0.12 to 0.81 (mean 0.54). Amplified microsatellite regions yield an average of 4.44 alleles per locus (ranging of 1 to 13). Cross-amplification to U. brizantha , U. humidicola and U. ruziziensis were successfully performed, although the number of the loci transferred varied among them. Multiple orrespondence Analysis revealed three distinct groups separating accessions and species. Four genetic markers presented high potential to distinguish sexual and apomictic accessions of Urochloa and must be further investigated. The genic markers identified in this study are the first set of expressed sequence tagged molecular markers for Urochloa species.
650 $aBrachiaria
653 $aSignalgrass
653 $aSimple Sequence Repeat
653 $aTransferability
700 1 $aCHIARI, L.
700 1 $aRESENDE, R. M. S.
700 1 $aVILELA, M. de M.
700 1 $aSALGADO, L. R.
773 $tAmerican Journal of Plant Science$gv. 18, n. 3, p. 314-319, July/Sept. 2018.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
19/12/2017 |
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Data da última atualização: |
21/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
REIS, A. N. dos; MIRANDA, M. dos S.; SILVA, L. K. X.; SILVA, A. O. A. da; SOUSA, J. S. de; MORAIS, E. de; GARCIA, A. R.; DOMINGUES, S. F. S.; SILVA, J. K. do R. da; RIBEIRO, H. F. L. |
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Afiliação: |
Adriana Novaes dos Reis, UFPA; Moysés dos Santos Miranda, UFPA; Lílian Kátia Ximenes Silva, UNAMA; Aluizio Otavio Almeida da Silva, UFPA; José Silva de Sousa, UFPA; Eziquiel de Morais, IFPA; ALEXANDRE ROSSETTO GARCIA, CPPSE; Sheyla Farhayldes Souza Domingues, UFPA; Joyce Kelly do Rosário da Silva, UFPA; Haroldo Francisco Lobato Ribeiro, UFRA. |
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Título: |
Comparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 38, n. 6, p. 3613-3628, 2017. |
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DOI: |
10.5433/1679-0359.2017v38n6p3613 |
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Idioma: |
Inglês |
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Conteúdo: |
For artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evaluated sperm membrane integrity with the fluorescent probes PI, FITC-PSA and JC-1 using flow cytometry. The data were submitted to ANOVA, and the results were compared by Tukey?s test at a significance of 5%. In the first experiment, the extender TES-TRIS showed better results for the various characteristics evaluated compared to ACP-112® and ACP-112®-Milk (P < 0.05), demonstrating greater protection of the buffalo sperm structures during cryopreservation. In the second experiment, the treatments with different concentrations of Lippia origanoides essential oil extract showed no differences among the assessed variables regarding the protection of sperm structures during cryopreservation (P > 0.05). Based on these data, we demonstrated the beneficial effects of TES-TRIS for post-thaw buffalo sperm quality; however, no protective effect was observed for buffalo sperm cryopreserved with the different tested concentrations of Lippia origanoides extract oil. MenosFor artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evalu... Mostrar Tudo |
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Palavras-Chave: |
Powdered Coconut Water. |
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Thesaurus NAL: |
cryopreservation; Lippia. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169304/1/REIS-et-al-2016-Comparative-Evaluation-Between-TES-TRIS-and-ACP-112.pdf
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Marc: |
LEADER 03425naa a2200277 a 4500
001 2082949
005 2019-05-21
008 2017 bl uuuu u00u1 u #d
024 7 $a10.5433/1679-0359.2017v38n6p3613$2DOI
100 1 $aREIS, A. N. dos
245 $aComparative evaluation between the extenders TES-TRIS and ACP-112® and the association of Sálva Marajó oil (Lippia origanoides) in the quality of cryopreserved buffalo sperm.$h[electronic resource]
260 $c2017
520 $aFor artificial insemination, it is essential to use frozen semen, however the freezing process causes deleterious changes to the structure and integrity of sperm membranes that compromise the function of sperm. To avoid this cellular damage, extenders and suitable substrates must be used to recover the highest possible number of viable cells post-thaw. To this end, in the first experiment, we evaluated three different extenders: TES-TRIS, which is widely used for buffaloes; and an extender composed of powdered coconut water-based (ACP-112®) with or without milk (ACP-112®-milk) for buffalo semen freezing. In the second experiment, we evaluated the effect of Lippia origanoides oil extract on protecting buffalo sperm against cryoinjury arising from freezing semen. Semen was collected from ten buffalo bulls (10 ejaculates/bull) and diluted in TES-TRIS (control), ACP-112® or ACP-112®-Milk in the first experiment. In the second experiment, the samples were diluted in the diluent with the best results for sperm quality obtained in experiment I, and 2.5 µg mL-1, 5 µg mL-1 or 10 µg mL-1 of the plant extract was added to treatments; and a control group containing only the diluent was also included. The fresh semen was analyzed for conventional features such as motility, concentration, morphology and viability. After thawing, the samples were evaluated again for motility, vigor and supra-vital staining, and then, were performed the of thermal-resistance test, hypoosmotic test and evaluated sperm membrane integrity with the fluorescent probes PI, FITC-PSA and JC-1 using flow cytometry. The data were submitted to ANOVA, and the results were compared by Tukey?s test at a significance of 5%. In the first experiment, the extender TES-TRIS showed better results for the various characteristics evaluated compared to ACP-112® and ACP-112®-Milk (P < 0.05), demonstrating greater protection of the buffalo sperm structures during cryopreservation. In the second experiment, the treatments with different concentrations of Lippia origanoides essential oil extract showed no differences among the assessed variables regarding the protection of sperm structures during cryopreservation (P > 0.05). Based on these data, we demonstrated the beneficial effects of TES-TRIS for post-thaw buffalo sperm quality; however, no protective effect was observed for buffalo sperm cryopreserved with the different tested concentrations of Lippia origanoides extract oil.
650 $acryopreservation
650 $aLippia
653 $aPowdered Coconut Water
700 1 $aMIRANDA, M. dos S.
700 1 $aSILVA, L. K. X.
700 1 $aSILVA, A. O. A. da
700 1 $aSOUSA, J. S. de
700 1 $aMORAIS, E. de
700 1 $aGARCIA, A. R.
700 1 $aDOMINGUES, S. F. S.
700 1 $aSILVA, J. K. do R. da
700 1 $aRIBEIRO, H. F. L.
773 $tSemina: Ciências Agrárias, Londrina$gv. 38, n. 6, p. 3613-3628, 2017.
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Embrapa Pecuária Sudeste (CPPSE) |
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